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Mouse colonoids Three-day-differentiated colonoids in 48-well plates were washed 3 times then starved 30 min in glucose-free Hepes buffer consisting of 120 mmol/l NaCl, 5 mmol/l KCl, 2.2 mmol/l NaHCO 3 , 1 mmol/l MgCl 2 , 2 mmol/l CaCl 2 and 10 mmol/l Hepes, supplemented with 0.1% BSA (pH = 7.2–7.4)
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