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Methods Cell culture INS-1 832/3 rat insulinoma cells were maintained in RPMI-1640 at 11 mM D-glucose, supplemented with 10% foetal bovine serum (FBS), 10 mM HEPES, 2 mM L-glutamine, 1 mM pyruvate, 50 μM β-mercaptoethanol and 1% penicillin/streptomycin
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