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[DOI] [PMC free article] [PubMed] [Google Scholar] 9.Müller T.D., et al
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Although high concentrations (500 μmol/l) of metformin were required to observe AMPK activation after brief (1 h) treatment of cells, significant effects were observed after incubation for much longer periods with just 20 μmol/l metformin, more compatible with concentrations of the drug found in the portal vein