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For the tirzepatide–GIPR–G s complex, cell pellets were lysed in a buffer containing 20 mM HEPES, 100 mM NaCl, pH 7.4, 10 mM MgCl 2 , 1 mM MnCl 2 and 10% glycerol supplemented with protease inhibitor cocktail, EDTA-free (TragetMol)

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Tailoring doses to individual needs could lead to better outcomes for those struggling with sugar cravings